Table 1. Summary of corneal endothelial morphology at older ages in mice lacking superoxide dismutase 1 and/or 3 and in C57BL/6 wild type controls.
| Mouse type | Age (months) | A (µm2) | HSF (A.U.) | DE (A.U.) | P (µm) | CV | n= |
|---|---|---|---|---|---|---|---|
|
Wild type |
10.4±3.0 |
246±35 |
1.30±0.40 |
0.19±0.02 |
58±4 |
0.35±0.027 |
6 |
|
SOD-3 null |
10.9±3.0 |
353±56* |
1.35±0.23 |
0.19±0.02 |
69±6* |
0.35±0.064 |
6 |
|
SOD-1/3 null |
11.8±2.1 |
360±95* |
1.39±0.20 |
0.19±0.02 |
69±9* |
0.30±0.032 |
6 |
|
SOD-1 null |
12.1±0.0 |
273±25 |
1.35±0.24 |
0.19±0.02 |
61±3 |
0.36±0.018 |
6 |
|
Wild type |
61.8±18.5 |
494±57 |
1.30±0.32 |
0.14±0.02 |
84±5 |
0.24±0.036 |
36 |
|
SOD-3 null |
57.5±27.0 |
637±121* |
1.45±0.29 |
0.14±0.02 |
91±8* |
0.23±0.051 |
29 |
|
SOD-1/3 null |
49.9±5.8 |
619±67* |
2.21±0.75* |
0.19±0.06* |
98±6*# |
0.31±0.057*# |
21 |
| SOD-1 null | 64.9±22.3 | 504±74 | 1.36±0.27 | 0.14±0.02 | 83±6 | 0.26±0.038 | 32 |
A=mean cell area; HSF=hexagon shape factor (quantifying the deviation from the ideal hexagonal cell shape); DE=degree of cell elongation; P=mean cell perimeter; CV= coefficient of variation, expressing the degree of cell polymegethism; A.U.=arbitrary units. The asterisk indicates that there was significant difference from the corresponding wild type control, and the sharp (hash mark) signified that there was significant difference from the SOD-3 null genotype. The absence of SOD-3 leads to larger cells, and in the absence of SOD-3 and SOD-1, the cells also become more irregular with increased pleomorphism and polymegethism as the mice age.