Table 2. Measures of sample and data quality.
| Experiment #1 | Day 0 | Day 3 | Day 7 | Day 14 |
|---|---|---|---|---|
| GAPDH 5′/3′ ratios |
1.03 |
0.87 |
0.81 |
2.16 |
| Background |
43.41 |
55.04 |
37.47 |
39.53 |
| Noise (rawQ) |
1.34 |
1.69 |
1.16 |
1.19 |
| Scale factor |
1.765 |
1.524 |
2.785 |
2.586 |
| Percent present |
53.4 |
55 |
47.8 |
48 |
|
Experiment #2 |
Day 0 |
Day 3 |
Day 7 |
Day 14 |
| GAPDH 5′/3′ ratios |
1.11 |
0.79 |
1.03 |
2.44 |
| Background |
43.77 |
53.3 |
42.76 |
40.21 |
| Noise (rawQ) |
1.36 |
1.67 |
1.31 |
1.24 |
| Scale factor |
1.68 |
1.533 |
1.808 |
2.376 |
| Perecent present | 54.7 | 54.9 | 51.4 | 47.5 |
The quality of each chip was evaluated using standardized criteria including 5′/3′ ratio of GAPDH, background, noise (rawQ), scale factor, and percent present. All values met the required criteria.