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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: Cell Microbiol. 2008 Aug 15;10(11):2326–2338. doi: 10.1111/j.1462-5822.2008.01214.x

Fig. 5. The C-terminal truncated Ec-gp96 does not bind Stat3/phospho-Stat3 upon infection with OmpA+ E. coli.

Fig. 5

A. HBMEC expressing control plasmid, FL-Ec-gp96, Ec-gp96Δ200 or Ec-gp96ΔATP were infected with OmpA+ E. coli for various time points. Total lysates were then immunoprecipitated with anti-Ec-gp96 antibody followed by immunoblotting with antibodies to Stat3 or phospho-Stat3. Equal amounts of proteins (20 μg) from the lysates were also subjected to Western blotting with β-actin.

B. HBMEC expressing FL-Ec-gp96, Ec-gp96Δ200 and Ec-gp96ΔATP upon infection with OmpA+ E. coli for 30 min in eight-well chamber slides were stained with anti-phospho-Stat3 antibodies followed by Alexa 488 coupled secondary antibody. Actin was stained with rhodamine phalloidin. The cells were examined under a fluorescent microscope (magnification: 100×). Arrows indicate the bacteria attached to cells, the condensation of phospho-Stat3 or actin.