Figure 5.
Asn172 may contribute to Mg2+ coordination. (a) Mean G-V relationship of WT, N172D and N172K mutant channels in 0 and 10 mM [Mg2+]i. (b) Shifts of the G-V relationship caused by 10 mM [Mg2+]i for WT and Gln397, Tyr336, and Asn172 mutations. (c) Shifts of the G-V relationship caused by 10 mM [Mg2+]i. Inset, N172D may substitute other putative Mg2+ coordinates to enable Mg2+ binding.