Figure 4.

hDlg and PBK have slower migrating species that appear only at mitosis. Lysates of asynchronous (A) or synchronized HeLa cells at G₁-phase (G₁), S-phase (S), or mitosis (M) were separated by SDS/PAGE, then transferred to nitrocellulose. The blots were then probed with anti-hDlg (Left) or anti-PBK antibodies (Right). In both cases, slower migrating species of the proteins appear at mitosis. There was half as much of the asynchronous cell lysate as of the other lysates.