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. 2000 Apr 25;97(10):5167–5172. doi: 10.1073/pnas.090102397

Figure 7.

Figure 7

PBK is activated by a mitotic phosphorylation that can be induced by treating Sf9 cells with okadaic acid. Autoradiograph of an in vitro kinase assay: GST fusions of PBK (WT) or mutant PBK where lysines 64 and 65 were mutated to alanines (Mut) were purified from Sf9 cells treated (+ OA) or not (no OA) with 100 nM okadaic acid before lysis. These fusion proteins were used in in vitro autophosphorylation assays (GST-PBK, Left) or assays with myelin basic protein (MBP, Center) or histone (Right) as substrates.