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. 2008 Nov 17;3(11):e3742. doi: 10.1371/journal.pone.0003742

Figure 3. Binding and agonistic activity of patient α1-AAB and rabbit α1-AB.

Figure 3

Surface plasmon resonance sensorgrams depicting antibody binding to α1-AR peptides (A and B). Biotinylated peptide corresponding to the α1A-AR extracellular loop2 (eL2) were immobilized. A corresponding peptide to extracellular loop1 (eL1) served as negative control. α1-AAB from a patient with refractory hypertension (A) and rabbit α1-AB (B) bind exclusively to eL2. Note that the characteristics of the human antibody (A) and rabbit antibody (B) were the same. (C) Functional effects (cardiomyocyte contraction assay) of affinity-purified α1-AAB (0.1–1 µg/ml medium) against second extracellular loop from a patient with refractory hypertension and (D) rabbit α1-AB (0.12–1.2 µg/ml medium) against second extracellular loop. The functional findings corroborate the BIAcor results.