Skip to main content
. Author manuscript; available in PMC: 2009 Jul 10.
Published in final edited form as: Neuron. 2008 Jul 10;59(1):84–97. doi: 10.1016/j.neuron.2008.05.014

Figure 6. Acute blockade of Arc translation blocks mGluR induced LTD in CA1 neurons of acute hippocampal slices.

Figure 6

A,B Average normalized EPSC amplitudes of CA1 neurons from acute hippocampal slices recorded with pipettes containing 250 µM mismatch oligonucleotide (A) or Arc antisense oligonucleotide (B). DHPG (100 µM, 5 min) applied to the bath resulted in LTD of EPSC amplitudes in cells filled with mismatch oligonucleotide. In contrast, intracellular introduction of Arc antisense oligonucleotide via patch pipette blocks DHPG-induced LTD. N = # of neurons. C, Representative EPSCs from cells filled with Arc antisense oligonucleotide or mismatch oligonucleotide taken during pre-DHPG baseline (1) or 50 min after LTD induction (2; indicated in A and B). Scale bars = 50 pA/ 10 msec. D, LTD of EPSC amplitude (plotted as a percent of pre-DHPG baseline) at 60 min in Arc antisense oligonucleotide infused neurons that were paired with same day or same slice control neurons infused with mismatch oligonucleotide. Average of 7 experiments is plotted in bold.