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HPLC analysis of [f5U]ASL before (solid) and after (dashed) incubation with RluA and heat denaturation of the adduct. A, intact stem-loop showing an increased retention time for product [f5U]ASL. B, after digestion to free nucleosides; f5U elutes at 6.67 min, and the modified f5U product elutes at 2.64 min.
