Figure 3.

Fluorescence emission maxima, λ_max, of myristoylated MRP constructs (1 μM) containing a single tryptophan residue at various positions in the effector domain. The spectra were recorded in the absence (●) or presence (○) of 1.5 μM CaM. The results are averaged from six to nine experiments in the absence and five experiments in the presence of CaM. Errors bars along the y axis are shown and are not apparent if smaller than the size of the symbols. As controls, the λ_max of a peptide corresponding to binding site of myosin light chain kinase, which contains a single tryptophan residue at position 4, is shown for the free peptide (▾) as well as for the peptide bound to CaM (▿) (taken from ref. 24).