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. 1991 Aug;59(8):2555–2559. doi: 10.1128/iai.59.8.2555-2559.1991

Identification of Campylobacter jejuni and Campylobacter coli antigens with mucosal and systemic antibodies.

S J Wu 1, N D Pacheco 1, J J Oprandy 1, F M Rollwagen 1
PMCID: PMC258055  PMID: 1855976

Abstract

The development of a rapid and specific diagnostic assay for Campylobacter infections is important in determining the etiology of acute diarrhea in humans. Studies have shown that sonicated whole bacteria or partially purified antigens cross-reacted with antibodies against other closely related bacteria. To solve the problems of specificity, we identified specific antigens of Campylobacter jejuni and Campylobacter coli for use in diagnostic assays. We investigated the responses of serum, urine, and intestinal lavage antibodies in infected (fed live bacteria) and parenterally immunized (intraperitoneal injection of sonicated whole bacteria with adjuvant) mice directed against C. jejuni or C. coli by Western blot (immunoblot) analysis. Antibody responses were examined weekly for up to 28 days. Fewer antigens were detected by urinary and intestinal lavage fluid immunoglobulin A (IgA) than serum IgG and IgM for both parenterally immunized and infected mice. Serum from parenterally immunized mice detected more antigens than that from infected mice. Two high-molecular-weight antigens (62,000 and 43,000) were predominantly detected by serum, urine, and intestinal lavage fluids of both parenterally immunized and infected mice. Serum antibodies from 28-day parenterally immunized mice detected one antigen specific to C. coli with a molecular weight of 38,000 and one antigen specific to C. jejuni with a molecular weight of 27,000. An immunodominant protein with a molecular weight of 31,000 common to both C. jejuni and C. coli was also recognized by serum antibodies from parenterally immunized mice.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blaser M. J., Hopkins J. A., Berka R. M., Vasil M. L., Wang W. L. Identification and characterization of Campylobacter jejuni outer membrane proteins. Infect Immun. 1983 Oct;42(1):276–284. doi: 10.1128/iai.42.1.276-284.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blaser M. J., Reller L. B. Campylobacter enteritis. N Engl J Med. 1981 Dec 10;305(24):1444–1452. doi: 10.1056/NEJM198112103052404. [DOI] [PubMed] [Google Scholar]
  3. Burr D. H., Caldwell M. B., Bourgeois A. L., Morgan H. R., Wistar R., Jr, Walker R. I. Mucosal and systemic immunity to Campylobacter jejuni in rabbits after gastric inoculation. Infect Immun. 1988 Jan;56(1):99–105. doi: 10.1128/iai.56.1.99-105.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chowdhury M. N. Campylobacter jejuni enteritis; a review. Trop Geogr Med. 1984 Sep;36(3):215–222. [PubMed] [Google Scholar]
  5. Dubreuil J. D., Kostrzynska M., Logan S. M., Harris L. A., Austin J. W., Trust T. J. Purification, characterization, and localization of a protein antigen shared by thermophilic campylobacters. J Clin Microbiol. 1990 Jun;28(6):1321–1328. doi: 10.1128/jcm.28.6.1321-1328.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Elson C. O., Ealding W., Lefkowitz J. A lavage technique allowing repeated measurement of IgA antibody in mouse intestinal secretions. J Immunol Methods. 1984 Feb 24;67(1):101–108. doi: 10.1016/0022-1759(84)90089-9. [DOI] [PubMed] [Google Scholar]
  7. Harris L. A., Logan S. M., Guerry P., Trust T. J. Antigenic variation of Campylobacter flagella. J Bacteriol. 1987 Nov;169(11):5066–5071. doi: 10.1128/jb.169.11.5066-5071.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jones D. M., Eldridge J., Dale B. Serological response to Campylobacter jejuni/coli infection. J Clin Pathol. 1980 Aug;33(8):767–769. doi: 10.1136/jcp.33.8.767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kosunen T. U., Pitkänen T., Pettersson T., Pönkä A. Clinical and serological studies in patients with Campylobacter fetus ssp. jejuni infection: II. Serological findings. Infection. 1981;9(6):279–282. doi: 10.1007/BF01640991. [DOI] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Lane E. M., Batchelor R. A., Bourgeois A. L., Burr D. H., Olson J. G. Urine and faecal IgA response during naturally acquired infection with Campylobacter jejuni. Lancet. 1987 May 16;1(8542):1141–1141. doi: 10.1016/s0140-6736(87)91694-1. [DOI] [PubMed] [Google Scholar]
  12. Lee A., Logan S. M., Trust T. J. Demonstration of a flagellar antigen shared by a diverse group of spiral-shaped bacteria that colonize intestinal mucus. Infect Immun. 1987 Mar;55(3):828–831. doi: 10.1128/iai.55.3.828-831.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Logan S. M., Trust T. J. Molecular identification of surface protein antigens of Campylobacter jejuni. Infect Immun. 1983 Nov;42(2):675–682. doi: 10.1128/iai.42.2.675-682.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Logan S. M., Trust T. J. Outer membrane characteristics of Campylobacter jejuni. Infect Immun. 1982 Dec;38(3):898–906. doi: 10.1128/iai.38.3.898-906.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McDermott M. R., Bienenstock J. Evidence for a common mucosal immunologic system. I. Migration of B immunoblasts into intestinal, respiratory, and genital tissues. J Immunol. 1979 May;122(5):1892–1898. [PubMed] [Google Scholar]
  16. Mills S. D., Bradbury W. C., Penner J. L. Isolation and characterization of a common antigen in Campylobacter jejuni and Campylobacter coli. J Clin Microbiol. 1986 Jul;24(1):69–75. doi: 10.1128/jcm.24.1.69-75.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mills S. D., Kurjanczyk L. A., Penner J. L. Identification of an antigen common to different species of the genus Campylobacter. J Clin Microbiol. 1988 Jul;26(7):1411–1413. doi: 10.1128/jcm.26.7.1411-1413.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Newell D. G., McBride H., Pearson A. D. The identification of outer membrane proteins and flagella of Campylobacter jejuni. J Gen Microbiol. 1984 May;130(5):1201–1208. doi: 10.1099/00221287-130-5-1201. [DOI] [PubMed] [Google Scholar]
  19. Skirrow M. B. Campylobacter enteritis: a "new" disease. Br Med J. 1977 Jul 2;2(6078):9–11. doi: 10.1136/bmj.2.6078.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Svedhem A., Gunnarsson H., Kaijser B. Diffusion-in-gel enzyme-linked immunosorbent assay for routine detection of IgG and IgM antibodies to Campylobacter jejuni. J Infect Dis. 1983 Jul;148(1):82–92. doi: 10.1093/infdis/148.1.82. [DOI] [PubMed] [Google Scholar]
  21. Taylor D. E., Chang N. Immunoblot and enzyme-linked immunosorbent assays of Campylobacter major outer-membrane protein and application to the differentiation of Campylobacter species. Mol Cell Probes. 1987 Sep;1(3):261–274. doi: 10.1016/0890-8508(87)90038-7. [DOI] [PubMed] [Google Scholar]
  22. Thompson J. S., Cahoon F. E., Hodge D. S. Rate of Campylobacter spp. isolation in three regions of Ontario, Canada, from 1978 to 1985. J Clin Microbiol. 1986 Nov;24(5):876–878. doi: 10.1128/jcm.24.5.876-878.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Walker R. I., Caldwell M. B., Lee E. C., Guerry P., Trust T. J., Ruiz-Palacios G. M. Pathophysiology of Campylobacter enteritis. Microbiol Rev. 1986 Mar;50(1):81–94. doi: 10.1128/mr.50.1.81-94.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

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