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NAD(P)H quantitation in pancreatic β cells. (A) Increasing concentrations of purified NADH in deep-well slides were used to produce a standard fluorescence curve. (B) The whole-cell fluorescence signal of NAD(P)H in pancreatic islets at 1 mM and 20 mM glucose was determined in terms of NADH fluorescence from the linear equations of NADH standard curves as shown in A. Data are represented by the mean ± standard error (n = eight islets).
