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The dns-negative mutant is hypertransformable. The frequencies of chitin-induced transformation of a dns mutant and complemented strains were determined. Lane 1, A1552 (WT); lane 2, A1552/pBR322 (vector control); lane 3, A1552Δdns; lane 4, A1552Δdns/pBR322 (vector control); lane 5, A1552Δdns/pBR-Tet-VC0470-His (constitutive dns expression); lane 6, A1552Δdns/pBR-Promo-VC0470-His (indigenous promoter of dns). Results are from three or more independent experiments.
