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The extracellular nuclease is active in vitro. Supernatants from 2-hour-old bacterial cultures on chitin surfaces were collected and tested for nuclease activity by adding 4 μg of gDNA. After incubation at 30°C for 5 to 60 min (as indicated at the top of the lanes), DNA was reisolated, and its quality was visualized on agarose gels. The bacterial cultures used were A1552ΔhapR (A), A1552 (WT) (B), A1552ΔluxO (C), and A1552Δdns (D). Undegraded (arrow) and degraded (bar) gDNA is indicated.
