TABLE 1.
Enterococcus faecalis strains and plasmids used in this study
| Strain or plasmid | Relevant information | Reference or source(s) |
|---|---|---|
| Strains | ||
| V583 | E. faecalis | Clinical isolate |
| RR15 | RR15 insertion mutant of V583; Tetr | 19 |
| RR17 | RR17 insertion mutant of V583; Tetr | 19 |
| RR15-17 | RR15 RR17 double mutant insertion of V583; Tetr Cmr | This study |
| V583T | Insertion mutant of V583 with p3TET inserted between HK02 and the adjacent open reading frame encoding a putative lipoprotein | 19 |
| V583/pTCV-LacSpec | V583 containing pTCV-LacSpec; Spr | This study |
| V583/pTCV-RR17p | V583 containing pTCV-RR17p; Spr | This study |
| RR17/pTCV-LacSpec | RR17 containing pTCV-LacSpec; Spr Tetr | This study |
| RR17/pTCV-RR17p | RR17 containing pTCV-RR17p; Spr Tetr | This study |
| Plasmids | ||
| pTCV-Lac | Transcriptional lacZ fusion vector | 3 |
| pML28 | pAT28 with aphA3 promoter on a 369-bp EcoRI/BamHI fragment | 20 |
| pML28-RR17 | pML28 derivative containing E. faecalis RR17 coding sequence | This study |
| pTCV-LacSpec | SpcR derivative of pTCV-Lac | This study; Hancock and Perego, unpublished |
| pTCV-RR17p | pTCV-LacSpec derivative containing E. faecalis RR17 promoter; −722 to +122 | This study |