TABLE 2.
Primer | Sequencea |
---|---|
EFRR175′Nde | 5′-CGTGAAGCATATGGATGGACGAATTGTAATAG-3′ |
EFRR173′Xho | 5′-AATCGCTCGAGTTATTCATCATCCATTACAATC-3′ |
EFHK175′Nde | 5′-TGATGAACATATGAAACGATTAGAGCAATTATG-3′ |
EFHK173′Xho | 5′-CCTTTCTCGAGTCAATGAACAACATCACTGG-3′ |
RR17Bam25′ | 5′-GGGAGGATCCAAGCGGTGATTGAAGGTTT-3′ |
RR17SalI3′ | 5′-TTTAGTCGACTGGTGACATAATTGCTCTAATCG-3′ |
RR17Bam3′ | 5′- CGCGGATCCCAAAACCATCAGCTGCTTCA-3′ |
PduQEco5′ | 5′-CGCGAATTCCAAATCAAGCAACTCCGTCA-3′ |
Restriction sites are in boldface type.