TABLE 2.
Oligonucleotide primers used in this study
| Primer | Sequencea |
|---|---|
| EFRR175′Nde | 5′-CGTGAAGCATATGGATGGACGAATTGTAATAG-3′ |
| EFRR173′Xho | 5′-AATCGCTCGAGTTATTCATCATCCATTACAATC-3′ |
| EFHK175′Nde | 5′-TGATGAACATATGAAACGATTAGAGCAATTATG-3′ |
| EFHK173′Xho | 5′-CCTTTCTCGAGTCAATGAACAACATCACTGG-3′ |
| RR17Bam25′ | 5′-GGGAGGATCCAAGCGGTGATTGAAGGTTT-3′ |
| RR17SalI3′ | 5′-TTTAGTCGACTGGTGACATAATTGCTCTAATCG-3′ |
| RR17Bam3′ | 5′- CGCGGATCCCAAAACCATCAGCTGCTTCA-3′ |
| PduQEco5′ | 5′-CGCGAATTCCAAATCAAGCAACTCCGTCA-3′ |
a
Restriction sites are in boldface type.