TABLE 1.
List of C. perfringens isolates used in the study
| Strain | Type | Source and dated |
|---|---|---|
| Type B | ||
| NCTC8533 | etx (65), cpb2 (65), cpba | Lamb dysentery, UK, 1950s |
| Bar2 | etx (65), cpb2 (65), cpba | Sheep, Australia, 1980 |
| NCTC3110 | etx (65), cpb2 (65), cpba | NCTC, UK, unknown |
| CN677 | etx (65), cpb2 (65), cpba | Acute lamb dysentery, UK, 1942 |
| CN3447 | etx (65), cpb2 (65), cpba | Lamb dysentery, unknown, 1951 |
| CN1793 | etx (65), cpb2 (65), cpba | Toxigenic, UK, 1947 |
| CN2414 | etx (65), cpb2 (65), cpba | 3-day-old lamb, UK, 1948 |
| CN3425 | etx (65), cpb2 (65), cpba | Jejunum, 5-day-old lamb, UK, 1948 |
| JGS1984 | etx (65), cpb2 (65), cpba | Lamb, dysentery, unknown, 1996 |
| Type D | ||
| CN1020 | etx (48)b | Connaught Labs, Canada, 1944 |
| CN1675 | etx (NDc) | Ewe's udder, UK, 1946 |
| CN2062 | etx (48)b | Goat, fatal diarrhea, unknown, 1948 |
| CN3977 | etx (48)b | Lamb, carcass, UK, 1956 |
| CN3978 | etx (48)b | Lamb, carcass, UK, 1956 |
| CN3841 | etx (48)b | Ewe intestinal contents, unknown, 1955 |
| CN3793 | etx (48)b | NCTC8503, vaccine production strain, Kenya, 1955 |
| CN4031 | etx (65)b | Sheep carcass, UK, 1956 |
| CN1634 | etx (65)b | Lamb with suspected dysentery, UK, 1945 |
| CN2068 | etx (60), cpb2 (60)b,e | Lamb stomach, UK, 1948 |
| JGS1240 | etx (65), cpb2 (65)b,e | Sheep, bronchopneumonia and enterotoxemia, USA, 1992 |
| JGS1182 | etx (ND), cpb2 (ND)e | Sheep, sudden death and enterotoxemia, Canada, 1992 |
| CN1183 | etx (65), cpb2 (65), cpe (65)b | Lamb, UK, 1942 |
| CN3842 | etx (85), cpb2 (85), cpe (85)b | Ewe, small intestine, Spain, 1955 |
| CN4003 | etx (65), cpb2 (45), cpe (110)b | 3-day-old lamb, unknown, 1956 |
| CN3948 | etx (65), cpb2 (65), cpe (110)b | Sheep abomasums, Iran, 1956 |
| JGS1902 | etx (110), cpb2 (65), cpe (110)b | Sheep, enterotoxemia, USA, 1999 |
| JGS4138 | etx (110), cpb2 (65), cpe (110)b | Goat, sudden death, USA, 2002 |
| JGS4139 | etx (65), cpb2 (65), cpe (110)b | Goat, sudden death, USA, 2002 |
| JGS4152 | etx (110), cpb2 (65), cpe (110)b | Lamb, pulpy kidney, USA, 2002 |
a
The cpb plasmid size in type B or C isolates has not yet been evaluated.
b
Numbers in parentheses indicate the size of the plasmid (in kb) carrying the indicated toxin gene, as determined in a previous study (22), except the previous designations of some plasmids as 75 kb was changed to 65 kb to reflect their comigration with pCP8533etx, which was determined in this study to be 65 kb.
c
ND, not determined.
d
Abbreviations: UK, United Kingdom; USA, United States.
e
Carries type B etx plasmid (this study).