TABLE 2.
Kinetic parameters of M. tuberculosis thioredoxinsa
| Protein | Km (μM) | kcat (s−1) | kcat (s−1)/Km (M) |
|---|---|---|---|
| TrxAMtb | ND | ND | ND |
| TrxBMtb | 2.65 ± 0.07 | 1.79 ± 0.13 | (6.7 ± 0.03) × 105 |
| TrxCMtb | 2.05 ± 0.5 | 1.17 ± 0.24 | (5.75 ± 0.02) × 105 |
| TrxAEc | 2.8 ± 0.14 | 1.34 ± 0.12 | (4.7 ± 0.06) × 105 |
a
The assay was carried out as described in Materials and Methods. The apparent Km and apparent turnover (kcat) values were calculated by nonlinear regression using the Michaelis-Menten equation. Data are represented as means ± standard deviations. ND, not detected.