FIG. 6.

Yeast two-hybrid analysis of WXG100 protein interactions. The coding regions of WXG100 proteins were cloned in the bait (AD) and prey (BD) vectors, as indicated on the figure, and the resulting plasmids were transformed into a yeast strain bearing three reporters: ADE2, HIS3, and MEL1 (or lacZ). BD-EsxW and BD-EsxV exhibited intrinsic transactivating properties when fused to the GAL4BD and could not be used in this assay. M and H indicate medium and high stringency of growth, respectively, i.e., autotrophy for leucine-tryptophan (medium) or autotrophy for adenine-histidine-leucine-tryptophan plus the ability to use galactose (high). Combinations in panel A did not yield any productive interactions in these assays; data in panel B suggest positive interactions between EsxB-EsxW and EsxB-EsxB.