TABLE 1.
Plasmids used in this study
| Plasmid | Relevant description or genotypea | Reference or source |
|---|---|---|
| pOS1 | Multicopy shuttle vector | 26 |
| pTS1 | Temperature-sensitive shuttle vector | 15 |
| pKKUb-βGal | Ub14-Sacc | 4 |
| pKK172 | pTS1 carrying 1-kbp 3′ esxB-Km cassette and 1-kbp 5′ esxB | This study |
| pGG12 | pTS1 carrying 1-kbp 3′ esxW and 1-kbp 5′ esxW | This study |
| pGG42 | pTS1 carrying 1-kbp 3′ essC and 1-kbp 5′ essC | This study |
| pGG119 | pOS1 carrying pesxB and Ub-EsxB1-90 | This study |
| pGG120 | pOS1 carrying pesxB and EsxB1-90-Ub | This study |
| pGG141 | pOS1 carrying pesxB and Ub-EsxB1-85 | This study |
| pGG142 | pOS1 carrying pesxB and Ub-EsxB11-90 | This study |
| pGG165 | pOS1 carrying pesxB and Ub-EsxB1-81 | This study |
| pGG167 | pOS1 carrying pesxB and Ub-EsxB6-90 | This study |
| pGG196 | pOS1 carrying pesxB and EsxB1-44-Ub-EsxB45-90 | This study |
| pGG256 | pOS1 carrying pesxB and EsxB6-90 | This study |
| pGG258 | pOS1 carrying pesxB and EsxB11-90 | This study |
| pGG261 | pOS1 carrying pesxB and EsxB1-81 | This study |
| pGG262 | pOS1 carrying pesxB and EsxB1-85 | This study |
| pGADT7 | GAL4AD768-881 | Clontech |
| pGBKT7 | GAL4 DNA-BD1-147 | Clontech |
| pGG153 | pGADT7-GAL4 DNA-BD::esxW | This study |
| pGG155 | pGADT7-GAL4 DNA-BD::esxB | This study |
| pGG156 | pGADT7-GAL4AD::esxB | This study |
| pGG157 | pGADT7-GAL4AD::esxW | This study |
| pGG158 | pGADT7-GAL4AD::esxV | This study |
| pGG159 | pGBKT7-GAL4BD::esxV | This study |
| pGG182 | pGADT7-GAL4AD::esxL | This study |
| pGG183 | pGADT7-GAL4AD::esxP | This study |
| pGG184 | pGBKT7-GAL4BD::esxL | This study |
| pGG185 | pGBKT7-GAL4BD::esxP | This study |
| pGG157 | pGADT7-GAL4AD::esxW | This study |
| pEB199 | pOS1 carrying pesxB and EsxB | This study |
| pEB255 | pOS1 carrying pesxB and EsxB-His | This study |
| pUTE618 | Kan cassette | T. Koehler |
a
Numbers in subscripts indicate first and last amino acid encoded by DNA fragment cloned.