TABLE 1.
E. coli strains used in this study
| Straina | Relevant genotypeb | Reference or source |
|---|---|---|
| CC101 | F′CC101 | 7 |
| CC102 | F′CC102 | 7 |
| CC103 | F′CC103 | 7 |
| CC104 | F′CC104 | 7 |
| CC105 | F′CC105 | 7 |
| CC106 | F′CC106 | 7 |
| KA796 | ara thi Δ(prolac) | 45 |
| NR9163 | mutL218::Tn10 | 44 |
| NR9559 | mutL211::Tn5 | 9 |
| NR10836 | F′CC106 | 26 |
| NR11264 | recA56 srl-360::Tn10 | This work |
| NR11584* | zaj-403::Tn10 proC | This work |
| NR12403* | lacZ103 | This work |
| NR12404* | lacZ104 | This work |
| NR12406* | lacZ106 | This work |
| NR13138 | ara thi Δ(prolac) trpE9777 | 18 |
| NR13144 | ΔdinB::kan | NR13138 × P1/YG7207 |
| NR13145 | mutL::Tn10 | NR13138 × P1/NR9163 |
| NR13243 | F′CC103/dinB | This work |
| NR13244 | F′CC104/dinB | This work |
| NR13246 | F′CC106/dinB | This work |
| NR13436 | F′CC106 dgt::mini-Tn10cam | This work |
| NR16897 | F′CC101 mutL::Tn10 | NR13145 × F′CC101 |
| NR16898 | F′CC102 mutL::Tn10 | NR13145 × F′CC102 |
| NR16899 | F′CC103 mutL::Tn10 | NR13145 × F′CC103 |
| NR16900 | F′CC104 mutL::Tn10 | NR13145 × F′CC104 |
| NR16901 | F′CC105 mutL::Tn10 | NR13145 × F′CC105 |
| NR16902 | F′CC106 mutL::Tn10 | NR13145 × F′CC106 |
| NR16939 | Δdgt::cat | NR13138 × P1/Δdgt::cat |
| NR16942 | F′CC101 | NR13138 × F′CC101 |
| NR16943 | F′CC102 | NR13138 × F′CC102 |
| NR16944 | F′CC103 | NR13138 × F′CC103 |
| NR16945 | F′CC104 | NR13138 × F′CC104 |
| NR16946 | F′CC105 | NR13138 × F′CC105 |
| NR16947 | F′CC106 | NR13138 × F′CC106 |
| NR16948 | F′CC101 dgt | NR16939 × F′CC101 |
| NR16949 | F′CC102 dgt | NR16939 × F′CC102 |
| NR16950 | F′CC103 dgt | NR16939 × F′CC103 |
| NR16951 | F′CC104 dgt | NR16939 × F′CC104 |
| NR16952 | F′CC105 dgt | NR16939 × F′CC105 |
| NR16953 | F′CC106 dgt | NR16939 × F′CC106 |
| NR17040 | dinB F′CC103/dinB | NR13144 × F′/NR13243 |
| NR17041 | dinB F′CC104/dinB | NR13144 × F′/NR13244 |
| NR17042 | dinB F′CC106/dinB | NR13144 × F′/NR13246 |
| NR17044 | dinB dgt F′CC103/dinB | NR17040 × P1/NR16939 |
| NR17045 | dinB dgt F′CC104/dinB | NR17041 × P1/NR16939 |
| NR17046 | dinB dgt F′CC106/dinB | NR17042 × P1/NR16939 |
| NR17227 | F′CC101 mutL::Tn10 dgt | NR16948 × P1/NR9163 |
| NR17228 | F′CC102 mutL::Tn10 dgt | NR16949 × P1/NR9163 |
| NR17229 | F′CC103 mutL::Tn10 dgt | NR16950 × P1/NR9163 |
| NR17230 | F′CC104 mutL::Tn10 dgt | NR16951 × P1/NR9163 |
| NR17231 | F′CC105 mutL::Tn10 dgt | NR16952 × P1/NR9163 |
| NR17232 | F′CC106 mutL::Tn10 dgt | NR16953 × P1/NR9163 |
| NR17535* | lacZ103 mutL::Tn5 | NR12403 × P1/NR9559 |
| NR17536* | lacZ104 mutL::Tn5 | NR12404 × P1/NR9559 |
| NR17537* | lacZ106 mutL::Tn5 | NR12406 × P1/NR9559 |
| NR17538* | lacZ103 dgt | NR12403 × P1/NR16939 |
| NR17539* | lacZ104 dgt | NR12404 × P1/NR16939 |
| NR17540* | lacZ106 dgt | NR12406 × P1/NR16939 |
| NR17541* | lacZ103 mutL::Tn5 dgt | NR17535 × P1/NR9559 |
| NR17542* | lacZ104 mutL::Tn5 dgt | NR17536 × P1/NR9559 |
| NR17543* | lacZ106 mutL::Tn5 dgt | NR17537 × P1/NR9559 |
| NR17581 | F′CC103 recA56 | NR16944 × P1/NR11264 |
| NR17582 | F′CC103 dgt recA56 | NR16950 × P1/NR11264 |
| NR17583 | F′CC106 recA56 | NR16947 × P1/NR11264 |
| NR17584 | F′CC106 dgt recA56 | NR16953 × P1/NR11264 |
| NR17587 | F′CC104 recA56 | NR16945 × P1/NR11264 |
| NR17588 | F′CC104 dgt recA56 | NR16951 × P1/NR11264 |
| YG7207 | Δ(dinB-yafN)::kan | 23 |
With the exception of YG7207, all strains are also ara thi Δ(prolac) or derivatives of MG1655 (indicated with an asterisk). See Materials and Methods for details.
The designations F′CC101, F′CC102, F′CC103, F′CC104, F′CC105, and F′CC106 refer to the F′prolac originally present in strains CC101, CC102, CC103, CC104, CC105, and CC106, which permit measurement of A·T→C·G, G·C→A·T, G·C→C·G, G·C→T·A, A·T→T·A, and A·T→G·C reversions, respectively (7). The lacZ designations lacZ103, lacZ104, and lacZ106 refer to the lacZ alleles originally present on F′prolac in strains CC103, CC104 and CC106 but now located on the chromosome. The designations F′CC103/dinB, F′CC104/dinB, and F′CC106/dinB indicate deletion of the dinB gene on the F′ episome.