TABLE 3.
Mutant frequencies (mutants per 108 cells) for dgt strains with lac alleles residing on the E. coli chromosomea
Genotype | lac G·C→C·G | lac G·C→T·A | lac A·T→G·C |
---|---|---|---|
Wild type | 0.02 ± 0.02 | 0.3 ± 0.1 | 0.02 ± 0.02 |
dgt | 0.02 ± 0.02 | 0.3 ± 0.1 | 0.02 ± 0.02 |
mutL | 0.08 ± 0.03 | 0.9 ± 0.2 | 13.5 ± 0.9 |
dgt mutL | 0.09 ± 0.04 | 0.9 ± 0.2 | 16.9 ± 1.3 |
The wild-type strains used were NR12403, NR12404, and NR12406 for the three lac alleles, respectively; the dgt strains were NR17538, NR17539, and NR17540; the mutL strains were NR17535, NR17536, and NR17537; and the dgt mutL strains were NR17541, NR17542, and NR17543 (Table 1). Mutant frequencies were determined as described in Materials and Methods.