FIG. 4.

Results of DDAG assays in various irvR backgrounds. (A) Dextran T2000 was added to the wild-type strain UA159, the irvR mutant (GN01R), the irvR mutant strain harboring an empty shuttle vector (GN01Rs), the complemented irvR mutant (GN01Rc), and the irvR irvA double mutant (GN01RA). (B) The same experiment was performed using the wild-type strain UA159, the irvR mutant (GN01R), the gbpC mutant (LZ02C), and the gbpC irvR double mutant (GN01RC). Shown here are representative results seen after approximately 1 to 2 min of gentle agitation. These experiments were performed three times with similar results.