TABLE 1.
Bacterial strains and plasmids used in this study
| Strain/plasmid | Relevant characteristicsa | Reference/source |
|---|---|---|
| Strains | ||
| E. coli JM109 | e14−(McrA−) recA1 endA1 gyrA96 thi-1 hsdR17 (rK− mK+) supE44 relA1 Δ(lac-proAB) [F′ traD36proAB lacIqZΔM15] | Cloning strain |
| S. mutans | ||
| UA159 | Wild-type S. mutans | 1 |
| GN01R | UA159 ΔirvR Kmr | This study |
| GN01Rs | UA159 ΔirvR Kmr; pDL278 | This study |
| GN01Rc | UA159 ΔirvR Kmr; pDL278-irvR | This study |
| GN01RA | UA159 ΔirvA ΔirvR Kmr | This study |
| LZ02C | UA159 gbpC::pFW5 Spr | This study |
| GN01RC | UA159 ΔirvRgbpC::pFW5 Kmr Spr | This study |
| Plasmids | ||
| pBluescript | Cloning vector; Apr | Stratagene |
| pCR2.1 | Cloning vector; Apr Kmr | Invitrogen |
| pSC-A | Cloning vector; Apr | Stratagene |
| pFW5 | Suicide vector; Spr | 32 |
| pLZ02C | pFW5 gbpC internal fragment; Spr | This work |
| pGN01R | pFW5 allelic replacement of irvR; Spr | This work |
| pDL278 | E. coli-Streptococcus shuttle vector; Spr | 11 |
| pGN01Rc | pDL278 irvR+ Spr | This work |
| pGNaa3 | pSC-A aphAIII+ Apr Kmr | This work |
a
Apr, ampicillin resistance; Kmr, kanamycin resistance; Spr, spectinomycin resistance.