FIG. 3.

DNA affinity purification of GlnR. Magnetic beads coated with glnA, amtB, and amt1 promoter DNA were incubated with M. smegmatis cytoplasmic proteins. After washing steps, proteins bound to the DNA fragments were eluted using buffers containing different NaCl concentrations. Eluted proteins were separated by SDS-PAGE and subjected to tryptic in-gel digestion, MALDI-TOF MS, and peptide mass fingerprint analyses. The numbers indicate proteins identified by this approach, as shown in Table 1.