FIG. 5.

Real-time RT-PCR of the M. smegmatis wild-type strain. Cells were grown until the exponential growth phase was reached (OD₆₀₀, approximately 0.6 to 0.8). To induce nitrogen starvation, cells were harvested, washed, and resuspended in prewarmed Middlebrook 7H9 medium without a nitrogen source (−N); prewarmed standard Middlebrook 7H9 medium was used as a control (+N). RNA was isolated at the indicated time points, and transcription of amtB (open bars), amt1 (gray bars), and glnA (black bars) was monitored by real-time RT-PCR. For each gene the control value at zero time was defined as 1.