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Complementation of M. smegmatis glnR deletion strain MH1: hybridization of RNA isolated from M. smegmatis cells carrying control plasmid pMN016 or glnR delivery plasmid pMN016-glnR before (t0) and after induction of nitrogen starvation by addition of MSX. t15, 15 min after addition of MSX; t30, 30 min after addition of MSX; t60, 60 min after addition of MSX. (A) Transcription control for M. smegmatis glnR. (B) Transcription of glnA, amtB, and amt1.
