TABLE 1.
Proteins isolated by DNA affinity purificationa
| Accession no. | Protein | No. | Mol wt | Sequence coverage (%) | Promoter DNA |
|---|---|---|---|---|---|
| MSMEG_4954 | Transcription termination factor Rho | 01 | 71,762 | 60 | glnA |
| MSMEG_3081 | Hypothetical protein | 02 | 34,499 | 71 | glnA |
| MSMEG_5784 | GlnR | 03 | 27,933 | 39-60 | glnA |
| MSMEG_4954 | Transcription termination factor Rho | 04 | 71,762 | 60 | amtB |
| MSMEG_3081 | Hypothetical protein | 05 | 34,499 | 62-71 | amtB |
| MSMEG_5784 | GlnR | 06 | 27,933 | 47-54 | amtB |
| MSMEG_6157 | DNA topoisomerase I | 07 | 102,137 | 46 | amt1 |
| MSMEG_4954 | Transcription termination factor Rho | 08 | 71,762 | 37 | amt1 |
| MSMEG_5784 | GlnR | 09 | 27,933 | 26 | amt1 |
a
Identification of proteins after DNA affinity purification was carried out by performing tryptic in-gel digestion, MALDI-TOF MS, and peptide mass fingerprint analyses. The numbers are the same as the numbers in Fig. 3.