TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Sourcea or reference |
|---|---|---|
| Bacterial strains | ||
| O. tritici 5bvl1 | Wild type; Cr(VI)r | This study |
| O. triticiT | Type strain; Cr(VI)s | BCCM/LMG |
| O. tritici E117 | Mutant of 5bvl1; Tn5 inserted in chrA | This study |
| E. coli DH5α | φ80dlacZΔM15 endA1 recA1 gyrA96 thi-1 hsdR17(rK− mK+) relA1 supE44 deoR (lacZYA-argF)U169 | Promega |
| E. coli S17-1 λpir | Tpr SmrrecA thi pro hsdR hsdM+ RP4:2-Tc Mu:Km Tn7 λpir | Biomedal, Seville, Spain |
| E. coli AB1157 | F−thr-1 leuB6 proA2 his-4 thi-1 argE2 lacY1 galK2 rspL supE44 ara-14 xyl-15 mtl-1 tsx-33 | 17 |
| E. coli JI132 | As AB1157 but (sodA::Mud PR13)25 (sodB-Kan)1-Δ2 | 17 |
| O. triticiTchrFCAB | Type strain carrying chrF, chrC, chrA, and chrB genes from strain 5bvl1 | This study |
| O. triticiTchrCAB | Type strain carrying chrC, chrA, and chrB genes from strain 5bvl1 | This study |
| O. triticiTchrAB | Type strain carrying chrA and chrB genes from strain 5bvl1 | This study |
| O. triticiTchrB | Type strain carrying chrB gene from strain 5bvl1 | This study |
| O. tritici E117:chrA | Mutant of 5bvl1 complemented with the chrA gene cloned in pBBR1MCS-5 vector | This study |
| Plasmids | ||
| pSUP5011 | Tn5-based transposon | DSMZ |
| pGEM-T Easy | Apr; T-tailed PCR product cloning vector | Promega |
| pBBR1MCS-5 | Gmr; oripBBR1MCS Mob+lacZa, broad-host-range cloning and expression vector | 19 |
| pSJ3 | Apr; vector containing the promotorless lacZ | 4 |
| pUTmini-Tn5Tc | Tcr; vector containing the Tn5 transposon | Biomedal, Seville, Spain |
| chrp::lacZ | pSJ3 vector with chr promoter-lacZ fusion | This study |
| chrBp::lacZ | pSJ3 vector with promoter-chrB-lacZ fusion | This study |
| pTrc99A | Apr; expression vector | Amersham Pharmacia Biotech |
a
DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany; BCCM/LMG, Laboratorium voor Microbiologie, Universiteit Gent, Belgium.