FIG. 1.
The overexpression of pqsE in P. aeruginosa causes overproduction of pyocyanin. P. aeruginosa strains carrying either a vector plasmid (tube 1), a plasmid containing tacp-pqsE (tube 2), or a plasmid carrying a complementing genetic allele (pqsA for strain PJF-QA1 or pqsR for strain PJF-QR1) (tube 3) were grown in LB medium for 18 h, and aliquots of cultures were photographed to document the production of the blue-green pigment pyocyanin. Strain designations: PAO1, wild type; PJF-QE1, pqsE mutant; PJF-QA1, pqsA mutant; PJF-QR1, pqsR mutant; PDO111, rhlR mutant; and PDO100, rhlI mutant.
