Whole mount in situ hybridization for Slc12a1 (A–D), Barttin (E–H), Calb3 (I–L), Clck1a (M–P) and Slc12a3 (Q–T) in wild type E10.5 (A, E, I, M, Q) trunks, E11.5 (B, F, J, N, R) and E12.5 (C, G, K, O, S) IM and E15.5 (D, H, L, P, T) metanephros. Arrowheads indicate location of mesonephric tubules. ms – mesonephros, mt – metanephros.