Table 1.

PCR primers and restrictions enzymes used to analyze RP1L1

`Amplimer`	`Primer sequences 5′-3′ temperature`	`Annealing` `temperature`	`Product` `size (bp)`	`Restriction` `enzyme`
`2A`	`GCCAATCCCCCAAGCTG`	`60 °C`	`321`	`BamHI`
	`GGGTGTGGTGACAGAGCG`
`2B`	`CGTGCCTCTCTCCTTTGG`	`60 °C`	`444`	`HinfI`
	`AGGTCTAAAGAACCTTTTCAAGG`
`3`	`TGGTGAGACTGGATCCTTCC`	`60 °C`	`292`	`HpaII`
	`CAGCCCTACTGAACCACCAT`
`4A`	`CTGTTTTATTCCTTTATCCTGACGC`	`54 °C`	`417`	`AluI`
	`CTACCTCCCCCAGAACGG`
`4B`	`GCTTCCACCTGGTCGGCG`	`54 °C`	`386`	`EaeI`
	`GGCTGGGCTGGCACTGTC`
`4C`	`GGAAGAGGTGGGGACTGG`	`62 °C`	`411`	`DdeI`
	`TTGCCTTGCCTGGACAGC`
`4D`	`AGCGAATGGGGTGGGCGG`	`54 °C`	`404`	`MboII`
	`GAGTCCAGTGGGCTGTGG`
`4E`	`TCCCAGGCATTCTCACTACC`	`54 °C`	`362`	`BsaI`
	`AGCAGGAGTCGGATGTGTG`
`4F`	`CGGCCCCATACCTCCCCAC`	`60 °C`	`229`	`None`
	`TGAGCAGCAGTGGCTTCG`
`4G`	`GCCTCAGCCCCTCCTCACC`	`60 °C`	`392`	`RsaI`
	`TCCTCAAGGTCTTCTCCTCG`
`4H`	`CAGTGCCAGCCAGGGTGC`	`54 °C`	`207`	`None`
	`GTGGTCTCGTCCGCCAAC`
`4I`	`ATGGCTGGACAACATTCCA`	`54 °C`	`393`	`StuI`
	`ATCAGCGCCCTCATGATCT`
`4J`	`CCGGAGCAGACAGAGAGG`	`54 °C`	`410`	`BsaI`
	`CGTGAAGTTCTCCGTCATGG`
`4K`	`GATGTGACGTTGGGGAAGAC`	`54 °C`	`401`	`MslI`
	`AGCTAACTGCTCCAGGTTCG`
`4L`	`AGAGACAGTGAGGAGCAGAGG`	`60 °C`	`Variable`	`None`
	`TCTCCTTGCAGTCCTCCTTC`
`4M`	`GAGGGGGTGCAGTTAGAGG`	`62 °C`	`874`	`None`
	`CCTCGCAGGGACAGAACTC`
`4N`	`CTCTCCTTCACCCTGGAGG`	`65 °C`	`829`	`None`
	`CTTCTGACTCTGGCTGGACC`
`4O`	`GTAAAACGACGGCCAGTCCCCAGAGGCAGAAGGAG`	`69 °C`	`861`	`None`
	`CCTTTGTCGATACTGGTACTGCCTCTACACCTTCTGACTCAGG`
`4P`	`GAAGGGGAGGCCCAGAAG`	`62 °C`	`919`	`None`
	`CCAAGCTCGTGATTGTTTTC`

Amplimer 4N PCR product was sequenced using the following nested sequencing primers: 5′-CCT TCA CCC TGG AGG ACG-3′; 5′-GGC TGG ACC TCC CAT TC-3′. Amplimer 4O primers incorporated M13 sequences into the PCR products. M13 sequencing primers were then used to sequence this amplimer.