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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Fungal Genet Biol. 2008 Jul 15;45(10):1430–1438. doi: 10.1016/j.fgb.2008.07.004

Figure 2. Upc2p-GFPΔecm22 and Ecm22p-GFPΔupc2 localization shifts from membranes to perinuclear foci when cells are exposed to FLC.

Figure 2

Cells were grown +/−10 µg/mL FLC for 6 hours prior to visualization by fluorescence microscopy. GFP is shown in the left panel, Hoechst nuclear stain is shown in the middle panel and a merged image is shown on the right.

A. Ecm22p-GFP localization in the strain ECM22-GFPΔupc2 (Table 1, row 5) is diffusely punctate and nuclear associated in cells treated with FLC and membranous with some cells showing single punctate foci in untreated cells.

B. Upc2p-GFP localization is punctuate and nuclear associated in cells treated with FLC and membranous with some cells showing single punctate foci in the absence of FLC treatment in the strain UPC2-GFPΔecm22 (Table 1, row 4).

C. Punctate perinuclear localization is not within the nucleus. A representative plot of Upc2p-GFP in the strain UPC2-GFPΔecm22 grown in the presence of FLC demonstrates that peak GFP fluorescence is immediately adjacent to, but not within the nucleus. Nuclear associated fluorescence was analyzed by fluorescence intensity line profile. The fluorescence intensities of the GFP (protein fusion) and Hoechst (DNA stain) channels are measured along a line bisecting the nuclei. Fluorescence intensity is plotted on the Y-axis in arbitrary fluorescence units (A.F.U.s) and pixels are plotted on the X-axis.