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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Fungal Genet Biol. 2008 Jul 15;45(10):1430–1438. doi: 10.1016/j.fgb.2008.07.004

Figure 4. Upc2p-GFP and Ecm22p are induced in response to ergosterol biosynthesis inhibitors.

Figure 4

Figure 4

A. Upc2p-GFP and Ecm22p-GFP expression was measured by FACS after 6 H (white bars), 24 H (grey bars) and 48 H (black bars) of growth in increasing FLC concentrations. A wild type strain (s288C) was used as a control. Upc2p and Ecm22p expression was induced by FLC and increased over time.

B. Upc2p-GFP (black bars) and Ecm22p-GFP (gray bars) levels were measured by FACS after cells were treated with ergosterol biosynthesis inhibitors for 24 hours. s288C (white bars) was used as a no GFP control. Drugs that target ergosterol biosynthesis (terbinafine, lovastatin and itraconazole) increased expression of GFP fusion proteins, while drugs with targets outside the ergosterol biosynthetic pathway (SDS, amphotercin B and 5-FC) did not alter Upc2p-GFP and Ecm22p expression.