Figure 3.

WT and mutant (QPD and HPN) ERdj3 bind to D-Luc similarly and ATP does not affect their binding. Chemically denatured luciferase was used to coat the wells and the indicated amounts of WT (A) QPD (B), or HPN (C) ERdj3 were added to the wells with (hatched bars) or without (solid bars) ATP. The plates were developed with anti-ERdj3 as described in Figure 2. The arrow indicates the concentration of ERdj3 that was used in the following experiments.