Figure 5.

Only WT BiP releases ERdj3 from D-Luc. (A) Chemically denatured luciferase was added to the wells, which were then incubated with wild-type or mutant BiP. The binding of BiP to D-Luc was performed in the absence of ERdj3 with (hatched bars) and without ATP (solid bars) and detected with anti-BiP serum. (B, C) Denatured luciferase was added to wells and wild-type ERdj3 was allowed to bind as described. After washing, either wild-type or mutant BiP was added with (hatched bars) or without (solid bars) ATP. The amount of ERdj3 remaining was detected with an anti-ERdj3 antiserum (B) and the binding of BiP was detected with an anti-BiP antiserum (C) and expressed in OD units.