Figure 7.

Both BiP and ATP must be added simultaneously to induce the release of ERdj3 from substrate. Luciferase-binding assays were performed with wild-type ERdj3 or the QPD mutant. The indicated BiP proteins were added with no ATP (solid bars), with ATP as done in the previous experiments (stippled bars) or BiP was allowed to bind in the absence of ATP but after washing unbound BiP away, the D-Luc–ERdj3–BiP complexes were further incubated with ATP (hatched bars). The amount of ERdj3 remaining associated with D-Luc (A, B) and the amount of BiP in the D-Luc–wild-type ERdj3 complexes (C) were determined by ELISA.