TABLE 1.
Three proteins identified newly in otoliths by mass spectrometry
| Otolith source | Top database match | Mascot statistics | PEAKS/MS-BLAST Statistics | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Species | GenBank accession | Length (aa) | Number of Peps | Covg | Expecta | Number of Peps | Covg | Expectb | |
| Secreted acidic cysteine rich glycoprotein (Sparc) | |||||||||
| Trout | Trout | AAC99813.1 | 300 | 4 | 19% | 1.8 × 10−9 | 5 | 22% | 7.3 × 10−4 |
| Catfish | Zebrafish | NP_001001942.1 | 291 | 2 | 11% | 3.2 × 10−6 | 5 | 23% | 2.0 × 10−3 |
| Catfish | Catfishc | ESTs CF262751.1 & CV987488.1 | 291 | nd | nd | nd | 5 | 23% | 1.2 × 10−4 |
| Precerebellin-Like Protein (Cblnl) | |||||||||
| Trout | Trout | AAF04305.2 (isoform a) | 182 | 4 | 34% | 2.5 × 10−7 | 4 | 32% | 1.3 × 10−2 |
| Trout | Troutc | ESTs CX140456.1 & CX140457.1 (isoform b) | 206 | nd | nd | nd | 5 | 36% | 4.3 × 10−4 |
| Neuroserpin (SerpinI1) | |||||||||
| Trout | Zebrafish | XP_685811.1 | 448 | 3 | 9% | 7.4 × 10−4 | – | – | – |
| Trout | Medaka | ENSORLP00000015491d | 401 | – | – | – | 8 | 37% | 8.3 × 10−4 |
| Trout | Troutc | ESTs CA373728.1, CX251911.1, & CX251912.1 | ∼416 | nd | nd | nd | 9 | 37% | 6.9 × 10−8 |
aa Amino acids, Pep peptide, Covg coverage, nd not determined
aBased on the highest peptide MOWSE score
bBased on the protein BLAST E-value
cIdentified in post-hoc BLAST search
dIn Ensembl genome assembly HdrR