FIG. 1.

RT-PCR of synucleins within the Cochlea. RT-PCR was used to detect the presence of α-, β-, and γ-synuclein mRNA in microdissected rodent (rat—left panel, mouse—three central panels) cochlear tissue. Controls lacking reverse-transcriptase (denoted with a ‘-’) are included. Both an α- and γ-synuclein knockout (KO) mouse were also studied, demonstrating the correct phenotype of the null-mutant mouse. Quantitative PCR (qPCR—right panel) was used to assess relative mRNA differences of the three synucleins (α, β, γ) within microdissected cochlear tissue. WT Wild-type, KO knockout mice.