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. 2000 May 9;97(10):5220–5224. doi: 10.1073/pnas.97.10.5220

Figure 2.

Figure 2

GFP expression in GB133 cells. (A) Two-hybrid dependent expression of GFP in GB133 cells. Cells were transiently transfected with expression plasmids for a model bait (GAL4DB-fusion Smad4) and prey (MSG1) together with a β-galactosidase (β-Gal) reporter plasmid. Expression of GFP or β-galactosidase was evaluated 48 h after transfection. Wt + AD, wild-type prey with the transactivating domain; WtΔAD, wild-type prey lacking the transactivating domain; Mut + AD, prey mutant with the transactivating domain that does not interact with the bait. (B) GB133 cells maintain OriP-containing plasmids. Cells were transfected transiently with expression plasmids for GAL4DB or a GAL4DB-fusion CR2 transactivating domain (AD) with or without harboring the OriP sequence. GFP expression was evaluated 7 days after transfection, when cells formed confluent monolayers (phase contrast images are not shown).