Table 1.

Analysis of cell movement by using dias software

	Speed, μm/min	Directionality	Directional change, °	Roundness, %	Number of lateral pseudopodia per 10 min
Wild type	8.21  ± 1.81	0.85  ± 0.09	20.5  ± 5.8	51.7  ± 2.3	1.5  ± 0.5
Ddracgap1 null	4.9  ± 0.24	0.52  ± 0.04	46  ± 3.74	52  ± 1.2	10.3  ± 1.6
DdRac-1BQ61L	3.22  ± 0.58	0.24  ± 0.16	70.2  ± 12	54  ± 7.9	8.62  ± 1.7
DdRac-1BT17N	2.2  ± 0.25	0.012  ± 0.01	N/A	76.8  ± 13	N/A

Movement of cells was recorded by using National Institutes of Health image software (one image every 6 s). Cell movement was examined by tracing the movement of a single cell in a stack of images and analyzed by using dias software. Roundness is a measure (%) of how efficiently a given amount of perimeter encloses an area. A circle has the largest area for any given perimeter and has a roundness parameter of 100%. Generally, a more polarized cell shape produces less roundness. Directionality is the net path length divided by the total path length. This gives 1.0 for a completely straight path and a smaller value for a meandering path. Directional change represents the average angle of change in the direction of movement in each frame (6 s).