Figure 3.

Expression of LFA-1 mutants on COS cells and representative data for the ICAM-1 adhesion assay. Expression of the wild type and a subset of mutants that do not respond to mAb 240Q induction was determined by flow cytofluorometry. Data from a representative experiment are shown and were similar for all mutants and across all experiments. (A) Transfection efficacy, expressed as the percentage of cells staining positive for CD11a (TS1/22) and CD18 (TS1/18 and 240Q). (B) The relative expression levels of different mutants expressed as median MFI of CD11a and CD18 mAb. (C) Adhesion assay of COS cells expressing wild-type LFA-1 or LFA-1 with a D137A mutation in presence of Control mAb (1B7), Blocking mAb (TS1/22), or Activating mAb 240Q. The adhesion assay is representative of greater than seven independent experiments. Mean absorbency values and standard deviations from triplicate wells are shown.