Figure 3.

Dnmt3a induces CpG, CpA, and CpT methylation in Drosophila. NNA of genomic DNA extracted from wild-type (A, C, and E) and Dnmt3a-transgenic (B, D, and F) Drosophila. (A and B) FokI-digested DNAs labeled with [α-³²P]dGTP. (C and D) FokI-digested DNA labeled with [α-³²P]dATP. (E and F) MvaI-digested DNAs labeled with [α-³²P]dATP. In the MvaI experiments (E and F), a small amount of labeling Ap, Tp, and Gp can also be seen. This finding is attributable to background labeling at nicks in the DNA. The identities of the labeled nucleotides are shown in B and F.