Skip to main content
. 2000 May 9;97(10):5249–5254. doi: 10.1073/pnas.97.10.5249

Figure 1.

Figure 1

(A) Sid4p-HA localizes to the SPBs throughout the cell cycle. sid4-HA cells (KGY1576) were fixed and stained with DAPI, anti-HA, and anti-Sad1p. (B) Sid4p-HA SPB localization does not require intact microtubules. Before (a and b) or after (c–f) cold shock, KGY1576 cells were fixed and stained with DAPI and either TAT-1 or anti-HA. (C) Sid4p SPB localization is independent of the known proteins required for cytokinesis. Endogenous sid4-HA was visualized in spg1–106 (KGY1593), cdc7–24 (KGY1594), sid1–239 (KGY1591), sid2–250 (KGY1592), cdc11–123 (KGY1595), cdc14-118 (KGY1596), cdc15-140 (KGY1597), or cdc16-116 (KGY1598). Cells were grown to mid-logarithmic phase at 25°C, shifted to 36°C for 4 h, and fixed and stained with DAPI and anti-HA. (Bar = 10 μm.)