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. 2000 May 9;97(10):5249–5254. doi: 10.1073/pnas.97.10.5249

Figure 2.

Figure 2

Spg1p, Cdc7p, and Byr4p SPB localization requires Sid4p function. (A and B) spg1-HA sid4-SA1 (KGY1725; A) or cdc7-HA sid4-SA1 (KGY1600; B) cells were grown to mid-logarithmic phase at 25°C and then shifted to 36.5°C for 4 h (A) or 2 h (B). Cells were fixed and stained with DAPI, anti-HA, and anti-Sad1p. (C) sid4-SA1 (KGY1234) cells were grown to mid-logarithmic phase at 25°C and then shifted to 36.5°C for 4 h. Cells were fixed and stained with DAPI, anti-Byr4p, and TAT-1. (Bar = 10 μm.)