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Sid4p contains an SPB localization domain. Wild-type cells expressing the N-terminal 153 amino acids of Sid4p tagged with the HA epitope under control of the nmt1 promoter (pKG1534) were grown to mid-logarithmic phase under inducing conditions at 32°C. Cells were fixed and stained with DAPI, anti-HA to detect N153Sid4p-HA, and anti-Sad1p. (Bar = 10 μm.)
