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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Microb Pathog. 2008 Jul 4;45(4):282–289. doi: 10.1016/j.micpath.2008.06.003

Table 1. Effect of C-terminal mutations on the in vitro actin nucleation activity and in vivo virulence functions of IpaC.

Mutant Actin nucleationa Relative invasion (%)b Contact hemolysis (%)c
IpaC Yes 100±9 100±1
IpaCΔI Yes 0±0 8±3
IpaCΔIII No 0±0 6±3
IpaCΔ344-363 No 0±0 100±6
IpaCStag No 0±0 100±5
SipC Yes 0±0 10±5
a

This is an in vitro activity that involves purified protein and thus does not depend upon active type III secretion.

b

Invasion is relative to a positive control (SF621 complemented with ipaC), which gave 153±14 invading bacteria per well. (n=3)

c

Relative hemolysis is also related to SF621 complemented with ipaC. In this case, 100% is actually complete lysis of the RBCs. It is noteworthy that complete absence of ipaC still allows 5 to 10% hemolysis due to the residual activity of IpaB, which is inserted into the erythrocyte membranes even in the absence of IpaC.