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. Author manuscript; available in PMC: 2009 Oct 1.

Published in final edited form as: Microb Pathog. 2008 Jul 4;45(4):282–289. doi: 10.1016/j.micpath.2008.06.003

Table 2. The effect of scanning mutagenesis on the IpaC C-terminal tail on invasion.

345	363	Relative Invasion^a
SINQSKNSTASQIAGNIRA (wt)		100 ± 12
AINQSKNSTASQIAGNIRA		23 ± 14
SSNQSKNSTASQIAGNIRA		9 ± 7
SILQSKNSTASQIAGNIRA		73 ± 8
SINLSKNSTASQIAGNIRA		102 ± 5
SINQAKNSTASQIAGNIRA		100 ± 9
SINQSINSTASQIAGNIRA		95 ± 4
SINQSKASTASQIAGNIRA		95 ± 6
SINQSKNATASQIAGNIRA		93 ± 3
SINQSKNSAASQIAGNIRA		93 ± 15
SINQSKNSTLSQIAGNIRA		71 ± 15
SINQSKNSTASAIAGNIRA		101 ± 2
SINQSKNSTASQSAGNIRA		32 ± 15
SINQSKNSTASQISGNIRA		82 ± 38
SINQSKNSTASQIASNIRA		100 ± 10
SINQSKNSTASQIAGQIRA		90 ± 36
SINQSKNSTASQIAGAIRA		99 ± 5
SINQSKNSTASQIAGNSRA		97 ± 8
SINQSKNSTASQIAGNIRI		99 ± 10

^a

Invasion is relative to the positive control (SF621 + ipaC), which had 184±22 bacteria per well (n=3-5).