Table 3. Arginine 362 has a role in IpaC effector function.
Mutant | Relative invasiona |
---|---|
Wild-type | 100 ± 12 |
R362W | 3 ± 1 |
R362K | 118 ± 17 |
R362M | 66 ± 9 |
R362E | 32 ± 5 |
Invasion was tested using a standard gentamycin protection assay with SF621 complemented with wild-type ipaC serving as the positive control. Invasiveness by the positive control was 132± 16 bacteria per well. (n=3)