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. Author manuscript; available in PMC: 2008 Nov 7.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2006 Sep;47(9):3754–3765. doi: 10.1167/iovs.06-0207

Figure 2.

Figure 2

Sensitivity and specificity of IMPDH1 and IMPDH2 antibodies. Antibodies were tested against various concentrations of denatured recombinant canonical IMPDH1 and IMPDH2 proteins in standard Western analyses. Anti-N-IMPDH1 is IMPDH1 specific and anti-C-IMPDH2 is IMPDH2 specific. Anti-C-IMPDH1 had a much higher affinity (50×) for IMPDH1 than for IMPDH2. Anti-C-IMPDH1+2 can detect IMPDH1 and IMPDH2 with relative equal affinities. The distinguishable electrophoretic pattern of IMPDH1 and IMPDH2 recombinant proteins are due to variation in tag length.